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The species Chorisia glaziovii O. Kuntze is native to the northeast of Brazil, belongs to Bombacaceae family and has diversified uses in folk medicine, recovery of degraded areas and upholstery industry. The present work was realizated with the objective to determine the substrate type and temperature for conduction of germination tests with C. glaziovii seeds. The experiment was carried out in the Laboratory of Analysis of Seeds (CCA - UFPB), Areia City, Northeast of Brazil, in design completely randomized with the treatments distributed in outline factorial 4 x 4 (temperatures of 25, 30, 35 and 20-30°C and substrate towel paper, among blotting paper, between sand and between vermiculite). The following parameters were analyzed: germination percentage, first count germination, germination speed index, and length of seedlings. The temperature of 35°C was shown inadequate for conduction of germination and vigor tests of seeds of C. glaziovii, independently of the used substrates. It is recommended for conduction of the germination and vigor tests of the seeds of C. glaziovii the substrate between sand or towel paper, in the temperatures of 25 and 20-30°C.