MORPHOLOGICAL AND ISOZYMIC CHARACTERIZATION OF Pisolithus spp. ISOLATES

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Anderson Marcos de Souza
Dulcinéia de Carvalho
Sebastião Carlos da Silva Rosado
Nilza de Lima Pereira Sales

Abstract

Pisolithus spp. is a cosmopolitan fungus living in symbiosis with a number of tree species, mainly Eucalyptus and Pinus. Isolates from Brazil, USA and Canadá were used for the evaluation of the diversity of Pisolithus spp. Morphological characteristics and genetic marker were analysed for the identification of potential genotypes with broad genetic base that could be used in programs aiming at improving the ectomycorrhizal symbiosis. It was possible to group the isolates on the basis of morphological characteristics and genetic marker. The divergence rate for morphological characteristics ranged from 0 to 82% and for enzyme from 0 to 79%. The crosses apt to broaden the genetic base for morphological characteristics were P4 x P21 and P4 x P25 and enzymes were P13 x P18 and P15 x P33.

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