OPTIMIZING PALIAVANA SERICIFLORA BENTH. MICROPROPAGATION: SPECTRAL QUALITY AND BIODEGRADABLE MICROCONTAINERS AS CONSERVATION TOOLS
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Abstract
Background: Paliavana sericiflora, a vulnerable species. Brazilian endemic from the rupestrian fields, lacks established seedling production methods. Micropropagation offers a promising alternative for its conservation. This study evaluated the influence of spectral quality on in vitro multiplication, elongation, rooting and acclimatization in biodegradable microcontainer of P. sericiflora. Established shoots were subcultured in MS medium with plant growth regulators for bud multiplication under four spectral qualities: white, red, blue, and purple (red and blue combination 1:1). The same spectra were used in the elongation and rooting stages.
Results: All light spectra influenced bud multiplication. Buds with the highest vigour were produced under white, blue, and violet light, with values close to 1. Red light induced more elongated buds, but with lower vigour compared to the others. The elongation and rooting stages were most successful under purple or white light, yielding the highest number of roots approximately 12 roots per plant. The use of biodegradable microcontainers resulted in 100% survival, producing more vigorous plants for nursery hardening-off. Conclusion: Spectral quality had no significant effect on bud multiplication, but purple and white lights promoted better elongation, rooting, and plant vigour. These stages can be conducted simultaneously, optimizing time and development. Acclimatization was efficient, and seedlings were ready for hardening-off in approximately 110 days.
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