EFFECTS OF FOREST FRAGMENTATION ON NATURAL POPULATIONS OF Anadenanthera colubrina (Vell.) Brenan: INSIGHTS FOR CONSERVATION AND SUSTAINABLE MANAGEMENT
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Abstract
Background: The objective of this study is to characterize the diversity and genetic structure of A.
colubrina in three Atlantic Forest fragments in the south of Espírito Santo state, using Inter Simple
Sequence Repeats (ISSR) molecular markers. Genomic DNA from 85 trees was analyzed using 12
ISSR primers, generating 147 fragments, of which 109 were polymorphic (74%). Markers were
characterized as moderately informative, with a mean polymorphic informational content of 0.34.
Results: Low genetic diversity was found for the three fragments and in the total sample, based on
Nei’s diversity parameters (H* = 0.26) and Shannon’s index (I* = 0.38). In the dissimilarity analysis,
four groups were observed in the dendrogram with an exclusive clustering trend by location.
The analysis of molecular variance confirmed that most of the genetic variation is found within
populations (73.50%), however, with high genetic differentiation between them (ΦST = 0.26). This
result was supported by the Bayesian approach that indicated genetically structured populations.
Conclusion: The data obtained reveal that forest fragmentation affected the diversity and genetic
structure of A. colubrina and allow expanding knowledge for the development of effective strategies
for the conservation and management of the species.
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