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This work was conducted to investigate the using different organics substrate in the production of native seedlings fruits from Brazilian Savanna. The aim of this study was to evaluate the biometric parameters, nutrition and quality of Pouteria gardneriana seedlings produced in substrates containing different sources of organic matter. The following components were used to formulate the substrates: subsoil material (SB), rice husk (RH), cattle-manure compost [CC (corn silage+cattle manure)], cattle manure (CM), sugarcane bagasse (CB), sugar-mill filter cake (FC) and BioPlant (BP). Five substrates were formulated using proportions on a volume basis, as follows: SB+RH (1:1, v/v), SB+CC (1:1, v/v), SB+CM (3:1, v/v), CB+FC (3:2, v/v) and BP. Was evaluated stem length, root collar diameter, root dry weight, shoot dry weight and the Dickson quality index. The use of CM as a source of organic matter provided, in general, the best results for the biometric characteristics and dry matter production. The increase of organic matter in the substrate like SB+CM (3:1, v/v) and SB+CC (1:1, v/v) improved the soil quality and favored the growth and quality of seedlings guapeva, and can be more indicate for guapeva seedlings production as an alternative substrate.