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Barbatimão is an important native specie of Savannah, with economic potential and restoration of degraded areas, however, there are few agronomic studies with the specie, particularly regarding the fertilization with nitrogen and phosphorus in Cerrado soils. Thus, the objective was to study the initial growth, the levels of chlorophyll, dry matter production in function of N and P levels. The study was developed in 8 dm3 pots and kept in a greenhouse. There were studied four N levels (0, 20.82, 41.64 and 62.46 mg N kg-1) and four P levels (0, 41.72, 83.44 and 125.16 mg.kg-1 P2O5) in 4x4 factorial design in randomized blocks experimental design with four replications. After 210 days after sowing height, stem diameter, chlorophyll content, dry mass of shoot and root and leaf N content were measured. The nitrogen and phosphate application showed higher increment for all traits.