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Several factors are known to be responsible for the degradation of water quality in our Planet’s spring sources. The goal of this study was to evaluate the impact of different anthropogenic activities on physico-chemical and biological properties of five spring water located in Inconfidentes, Minas Gerais State, Brazil. Analytical results have demonstrated that water source protected by native vegetation had the highest quality in terms of color, turbidity, biological oxygen demand (BOD5), total phosphate, nitrate, dissolved oxygen (DO), fecal coliforms and thermo-tolerant coliforms. On the other hand, the water quality was negatively impacted by the lack of adequate agricultural practices, such as the use of chemical inputs, the nonexistence of fenced livestock grazing areas and residential sewage system which are considered to be indispensable practices to minimize the environmental impact of anthropogenic activities and to protect human health.