GROWTH AND SYMPTOMATOLOGICAL ASPECTS IN THE ACCLIMATIZATION OF IPÊ-ROXO

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João Paulo Rodrigues Martins Breno Régis Santos Sandro Barbosa Wesley Pires Flausino Máximo Luiz Alberto Beijo Renato Paiva

Abstract

This work evaluated the influence of seal type and substrate type on pre-acclimatization, and symptomatological aspects relating to acclimatization of ipê-roxo. For the pre-acclimatization, ipê-roxo plants obtained through an in vitro zygotic embryo culture were aseptically inoculated in test tubes with different types of seal (cotton plug, plastic cap + parafilm and plastic lid) and different substrates (agar, vermiculite and Plantmax®) supplemented with a WPM culture medium with 1 gL-1 of activated charcoal, 10 mgL-1 of citric acid and 30 gL-1 of sucrose added. For the acclimatization, in vitro ipê-roxo plants were transplanted into 56-cm3 plastic tubes containing vermiculite and Plantmax® at a 2:1 ratio which had been previously autoclaved at 121oC and 1 atm for 20 minutes. Once transplanted, the plants were irrigated with different concentrations of the MS medium (25%, 50%, 75%, 100% and 150%), using distilled water as control. After 30 days, the effects of seal type and substrate type on pre-acclimatization were examined. Cotton can be recommended where Plantmax® or vermiculite is used as substrate. In the acclimatization it was noted that where plants were irrigated with distilled water and concentrations < 50% they showed symptoms of nutritional deficiency. Similar symptoms were observed when using MS medium at a concentration equal to or greater than 100%. The 75% MS concentration was found to be the most effective at maintaining the nutritional vigor of ipê-roxo seedlings in the process of acclimatization.

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How to Cite
MARTINS, João Paulo Rodrigues et al. GROWTH AND SYMPTOMATOLOGICAL ASPECTS IN THE ACCLIMATIZATION OF IPÊ-ROXO. CERNE, [S.l.], v. 17, n. 4, p. 435-442, may 2015. ISSN 2317-6342. Available at: <http://cerne.ufla.br/site/index.php/CERNE/article/view/66>. Date accessed: 18 sep. 2019.
Keywords
Handroanthus impetiginosus, pre-acclimatization, tissue culture, seal
Section
Article