GERMINATION IN VITRO AND E EX VITRO OF EMBRYOS/SEEDS OF Tabebuia serratifolia (VAHL) NICH

Abstract

The knowledge of germination of seeds allows understanding the behavior of seeds during development and the conservation mechanism. For evaluating the germination of the seeds of ipê amarelo collected during seven development stages, 10, 18, 25 32, 39, 47 and 53 days after the anthesis, the medium of culture in vitro were tested, MS and WPM, and methods of disinfestations of the seeds in the germination ex vitro. Seeds of yellow ipê disinfestations with carbendazim (2% for 2 minutes), sodium hypochlorite (2% of the active chlorine for 3 minutes) and seeds without surface sterilisation, were submitted to the germination test on sand to 30 ºC, under constant white light. In the cultivation in vitro, seeds acquired germinative capacity 39 days after the anthesis and superior germination 53 days after the anthesis, independent of the medium of culture. In the germination ex vitro, the seeds disinfestations with carbendazim and without surface sterilization presented superior germination, the use of sodium hypochlorite caused fitotoxin effect to the seeds.