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Species of the genus Eucalyptus managed for sawlogs in fast-growing plantations show high potential for substitution for valuable native hardwoods. To obtain high quality wood, technical pruning is necessary. The objective of the study is to analyse the development of the branches for the first and second pruning lifts and to quantify the biomass of the pruned branches. For the study two stands of Eucalyptus grandis were selected (age 18 and 36 months) for evaluating a pruning lift from 0 to 3 m and 3 to 6 m. The average branch diameter and length were 18 mm and 2.1 m in the younger stand and 21 mm and 2.3 m in the older one. The relation between branch diameter and branch length could be expressed in a linear model (R² = 0.8). In both stands a higher proportion of branches were already dead. The oven-dry biomass of the pruned branches was 2.2 ton.ha-1 in the first pruning lift and 1.2 ton.ha-1 in the second. The results showed that branch development in wide spaced and early thinned eucalypt plantations is in line with the objective of high quality wood production. Pruning should take place before 18 month to avoid dead branches. The oven-dry branch biomass cut in the two pruning lifts shows a low volume making a commercial utilization difficult.