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This study was accomplished in two fragments of the gallery forest located in the Parque Canjerana in Brasília, DF, to characterize the structure and the floristic composition of the arboreal flora. Strips of 10 m of width, with varying lengths, depending on the limits of the forest, were systematically allocated perpendicularly to the water stream, distant 60 m from each other. Each strip was subdivided in continuous parcels of 10 x 20 m, so, in each fragment it was possible to allocate 14 parcels. The individuals with CHD> 5 cm were identified and had their values of total height and CHD registered. It was sampled a total of 747 alive individuals distributed in 79 species and 43 botanical families. The estimated forest basal area was approximately 31.29 m2.ha-1 and the density was 1,475 ind.ha-1. There was low similarity among the fragments. One of the fragments, denominated of fragment II, was characterized as a inundated gallery forest and the species Protium spruceanum and Tapirira guianensis were considered the most important. The other fragment, denominated fragment IV, was characterized as a non-inundated gallery forest and the species Anadenanthera colubrina and Qualea dichotoma stood out with high importance. The diversity of the fragment IV was larger than the one of the fragment II (index of Shannon=3.698 and 2.571nats.ind-1, respectively). The specificity of identified species in each fragment can be used to define restoration and conservation strategies of the Gallery Forest of the Parque Canjerana.