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Programs of integrated pest management in forest plantation require a sampling plan. Therefore, it is important to determine the spatial distribution these pests, because it makes the decision process more precise, given that it takes into account their local and regional spatial distribution variations. This work was carried out for evaluating the spatial distribution of ant nests in eucalyptus plantations. Twelve eucalyptus stands belonging to V&M Florestal Ltd., in Bocaiúva county, Minas Gerais, were used. They were divided into 6m x 20m sub parcels, totalizing 120m2. The nests were identified, counted and measured in each parcel. Both found and expected frequency of nests were calculated according to Poisson’s distribution, in order to determine the type of spatial distribution of nests. The found and the expected frequency (x2; p>0.01) were similar. The result was a randomized distribution.