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Tissue culture presents great potential for the establishment of native species in vitro, which have economic-ecological importance. The Celtis sp or "juazeiro-de-bode" is a semi-arid plant from Northeastern Brazil used as forage during the long dry season. Not much is known about its reproduction, and the micropropagation may represent one alternative for its propagation. The oxidation and contamination are the main problems in establishment, in vitro, of any part of plants. For studying the in vitro establishment of Celtis sp the following experiments were done: a) nodal cutting obtained in water, b) shoots dipped in benlate and c) apical and lateral shoots treated with benlate at different concentration. The Celtis sp exhibited resistance to oxidation; the washing in water favored the fungi infection; cuttings treated in benlate at 200mgL-1 concentration inhibited the fungi growth, without causing phytotoxic effect.