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The aim of this study was to determine the optimum plot size as well as the appropriate sample size in order to provide an accurate sampling of natural regeneration surveys in high floodplain forests, low floodplain forests and in floodplain forests without stratification in the Amazonian estuary. Data were obtained at Exportadora de Madeira do Pará Ltda. – EMAPA forestlands, located in Afuá County, State of Pará. Based on the results, the following plot sizes were recommended: 70m2 - SC1 (0,3m ≤ h < 1,5m), 80m2 - SC2 (h ≥ 1,50m to DAP < 5,0cm), 90m2 - SC3 (5,0cm ≤ DAP < 15,0 cm) and 70m2 – ASP (h ≥ 0,3m to DAP < 15,0cm. Considering these optimum plot sizes, it is possible to obtain a representative sampling of the floristic composition when using 19 sub-plots in high floodplain, 14 sub-plots in low floodplain, and 19 sub-plots in the forest without stratification to survey the species of SC1 and the species of all sampled population (ASP), while 39 sub-plots are needed for sampling the natural regeneration species in SC2 and SC3.