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Increasing knowledge about tree production practices to help forest restoration projects is essential but still lacking for many tree species. Maytenus boaria is a neotropical tree distributed across the temperate and subtropical South American mountains. In central Argentina, is mainly restricted to the most preserved forest remnants. Attempts to plant this species have had little success due to difficulties in seedling production and low survival. We set up four trials aiming to identify the constraints of seedling production and outplanting. Under greenhouse conditions, we evaluated (i) pre–germination treatments and (ii) seedling response to inoculation with arbuscular mycorrhizal fungi (AMF). In the field, we planted saplings together with saplings of the most abundant tree in our study site and recorded (iii) survival and height for 10 years. Finally, (iv) we quantified natural recruitment in an attempt to determine M. boaria regeneration niche. Germination varied from 13.1 to 29.2%; stratification at 5 ºC significantly improved seed germination. Shoot phosphorus concentration in AMF-treated seedlings was significantly higher (45%) than in non-inoculated seedlings. Survival of M. boaria saplings was similar to that of the most abundant tree in our study site, but their lower height suggested limited growth. We recorded low abundance of M. boaria seedlings in the field; therefore, we were unable to identify the characteristics of it regeneration niche. Our results show how the utilization of disparate techniques under controlled and field conditions could improve the knowledge about the performance of tree species.