Neotropical tree production: insights into germination, growth and outplanting for Maytenus boaria

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Nicolás Marro Florencia Soteras Noelia Cofré Ignacio Ibarra Romina Torres Alejandra Gabriela Becerra Daniel Renison


Increasing knowledge about tree production practices to help forest restoration projects is essential but still lacking for many tree species. Maytenus boaria is a neotropical tree distributed across the temperate and subtropical South American mountains. In central Argentina, is mainly restricted to the most preserved forest remnants. Attempts to plant this species have had little success due to difficulties in seedling production and low survival. We set up four trials aiming to identify the constraints of seedling production and outplanting. Under greenhouse conditions, we evaluated (i) pre–germination treatments and (ii) seedling response to inoculation with arbuscular mycorrhizal fungi (AMF). In the field, we planted saplings together with saplings of the most abundant tree in our study site and recorded (iii) survival and height for 10 years. Finally, (iv) we quantified natural recruitment in an attempt to determine M. boaria regeneration niche. Germination varied from 13.1 to 29.2%; stratification at 5 ºC significantly improved seed germination. Shoot phosphorus concentration in AMF-treated seedlings was significantly higher (45%) than in non-inoculated seedlings. Survival of M. boaria saplings was similar to that of the most abundant tree in our study site, but their lower height suggested limited growth. We recorded low abundance of M. boaria seedlings in the field; therefore, we were unable to identify the characteristics of it regeneration niche. Our results show how the utilization of disparate techniques under controlled and field conditions could improve the knowledge about the performance of tree species.

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MARRO, Nicolás et al. Neotropical tree production: insights into germination, growth and outplanting for Maytenus boaria. CERNE, [S.l.], v. 23, n. 3, p. 377-385, oct. 2017. ISSN 2317-6342. Available at: <>. Date accessed: 18 dec. 2017.
high mountain forests; management; mycorrhizal inoculation; reforestation


Cabido, M. Las comunidades vegetales de Pampa de Achala, Sierras de Córdoba, Argentina. Documents Phytosociologiques, v. 9, p. 431-443, 1985.
Cingolani, A, Renison, D.; Zak, M. R.; Cabido, M. R. Mapping vegetation in a heterogeneous mountain rangeland using landsat data: an alternative method to define and classify land-cover units. Remote Sensing of Environment, v. 92, p. 84-97, 2004.
Correa, M. N. Flora Patagonica. Parte V. Dicotiledoneas a dialipetalas (Oxalidaceae a Cornaceae). Argentina, Buenos Aires. Instituto Nacional de Tecnología Agropecuaria,1988.
Donoso, C. Z. Las especies arbóreas de los bosques templados de Chile y Argentina: Autoecología. Valdivia, Chile. María Cuneo Ediciones, 2006, 678p.
Donoso, C.; Wendler, J. Antecedentes morfológicos y genecológicos. Bosque, v 6, p. 93–99, 1985.
Ellenberg, H. Man’s influence on tropical mountain ecosys- tems in South America. Journal of Ecology, v. 67, p. 401–416, 1979.
Fontenla, S.; Godoy, R.; Rosso ,P.; Havrylenko ,m. Root associations in Austrocedrus forests and seasonal dynamics of arbuscular mycorrhizas. Mycorrhiza, v. 8, p. 29-33,1998.
Hensen, I. Impacts of anthropogenic activity on the vegetation of Polylepis woodlands in the region of Cochabamba/Bolivia. Ecotropica, v. 8, p. 183–203, 2002.
Herrera, C. M.; Jordano, P.; Lopez-Soria, L.; Amat, J. A. Recruitment of a mast-fruiting, bird-dispersed tree: Bridging frugivore activity and seedling establishment. Ecological Monographs, v. 64, p. 315-344, 1994.
Janos, D. P. Mycorrhizae Influence Tropical Succession. Biotropica, v. 12, n. 2, p. 56–64, 1980.
Jones J. B.; Wolf B.; Mills H. A. Plant analysis handbook. A practical sampling, preparation, analysis, and interpretation guide. Athens, Micro-Macro Publishing Inc,1991. 213 p.
Kauffman J. B.; Steele M. D.; Cummings D. L.; Jaramillo V.J. Biomass dynamics associated with deforestation, fire, and conversion to cattle pasture in a Mexican tropical dry forest. Forest Ecology and Management, v. 176, p. 1-12, 2003.
Kaye, T.; Kuykendall, K. Effects of scarification and cold stratification on seed germination of Lupinus sulphureus ssp. kincaidii. Seed Science Technology, v. 29, p. 663-668, 2001.
Marcora, P. I.; RenisoN, D.; País-Bosch, A. I.; Cabido, M. R.; Tecco, P. A. The effect of altitude and grazing on seedling establishment of woody species in central Argentina. Forest Ecology and Management, v. 291, p. 300-307, 2013.
Marro, N.; Lax, P.; Cabello, M.; Doucet, M. E.; Becerra, A. G. Use of the arbuscular mycorrhizal fungus Glomus intraradices as biological control agent of the nematode Nacobbus aberrans parasitizing tomato. Brazilian Archives of Biology and Technology, v. 57, p. 668-674, 2014.
McGonigle T. P.; Millers, M. H.; Evans, D.G.; Fairchild, G. L.; Swan, J. A. A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytologist, v. 115, p. 495-501, 1990.
Phillips, J. M; Hayman, D. S. Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, v. 55, p.158-161, 1970.
R Core Team, 2013. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
Reid, S.; Armesto, J. Avian gut-passage effects on seed germination of shrubland species in Mediterranean central Chile. Plant Ecology, v. 212, n. 1, p. 1-10, 2011a.
Reid, S.; Armesto, J. 2011b. Interaction dynamics of avian frugivores and plants in a Chilean Mediterranean shrubland. Journal of Arid Environment, v. 75, n. 3, p. 221-230.
Renison, D.; Cuyckens , G. A. E.; Pacheco, S.; Guzmán, G. F.; Grau, H. R.; Marcora, P.; Robledo, G. L.; Cingolani, A. M.; Dominguez, J.; Landi, M.; Bellis, L.; Hensen, I. Distribución y estado de conservación de las poblaciones de árboles y arbustos del género Polylepis (Rosaceae) en las montañas de Argentina. Ecología Austral, v. 23, p. 27-36, 2013.
Renison D.; Chartier, M. P.; Menghi, M.; Marcora, P. I.; Torres, R. C.; Giorgis, M.; Hensen, I.; Cingolani, A. M. Spatial variation in tree demography associated to domestic herbivores and topography: Insights from a seeding and planting experiment. Forest Ecology and Management, v. 335, p.139-146, 2015.
Renison, D.; Hensen, I.; Suarez, R. Landscape structural complexity of high-mountain Polylepis australis forests: a new aspect of restoration Goals. Restoration Ecology, v. 19, n. 3, p. 390-398, 2011.
Samuels I. A.; Levey, D. J. Effects of gut passage on seed germination: do experiments answer the questions they ask? Functional Ecology, v. 19, n. 2, p. 365-368, 2005.
Smith S. E.; Jakobsen, I.; Gronlund, M.; Smith, F. A. Roles of arbuscular mycorrhizas in plant phosphorus nutrition: Interactions between pathways of phosphorus uptake in arbuscular mycorrhizal roots have important implications for understanding and manipulating plant phosphorus acquisition. Plant Physiology, v. 156, n. 3, p. 1050-1057, 2011.
Therneau, T. M. 2014. A Package for Survival Analysis in S.
Torres, R. C.; Renison, D.; Hensen, I.; Suarez, R.; Enrico, L. Polylepis australis’ regeneration niche in relation to seed dispersal, site characteristics and livestock density. Forest Ecology and Management, v. 254, p. 255-260, 2008.