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The objective of this research was to determine the more appropriate moisture content to the cryoconservation of the “yellow ipe” seeds when they are submitted to storage at the temperature of - 196°C per five days. Samples of yellow ipe seeds were prepared with moisture content between 4 and 12%, wb. Test of germination were conducted and vigor of the seeds measured before and after the cryoconservation. The limit moisture content obtained was 4% w.b. for the cryoconservation of the yellow ipe seeds.