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The knowledge about the influence of habitat variables is essential to understand the underlying ecological patterns in vegetation. This study compared the floristic composition of two forest communities located in different altitudes. Associated with this comparison, we used a methodology where habitat descriptor variables were scaled and interpreted by the biotic set sampled. We constructed one matrix with scores given to physical, biotic, vegetation, and anthropogenic variables in the field and one matrix with the species sampled and performed multivariate analyses. We found that the floristic communities differ between the different altitudes and that the methodology used showed significant variables for the ecological characterization of the sampled habitat.